Recombinant Avian infectious bronchitis virus Non-structural protein 5a (5a)
Product Specifications
Product Name Alternative
(ns5a) (Accessory protein 5a)
Abbreviation
Recombinant Avian infectious bronchitis virus Non-structural protein 5a
Gene Name
5a
UniProt
Q89613
Expression Region
1-65aa
Organism
Avian infectious bronchitis virus (strain Beaudette) (IBV)
Target Sequence
MKWLTSFGRAVISCYKSLLLTQLRVLDRLILDHGLLRVLTCSRRVLLVQLDLVYRLAYTPTQSLA
Tag
C-terminal 6xHis-sumostar-tagged
Type
Developed Protein
Source
Yeast
Field of Research
Microbiology
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
20.3 kDa
References & Citations
"S gene and 5a accessory gene are responsible for the attenuation of virulent infectious bronchitis coronavirus." Zhao Y., Cheng J., Yan S., Jia W., Zhang K., Zhang G. Virology 533:12-20 (2019)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length
Available Sizes
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