Anti-F1Aa Antibody

Rabbit Polyclonal Antibody specific to F1A α (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

FEM1b, FEM1-beta, Fem-1-like death receptor-binding protein α, Fem-1-like in apoptotic pathway protein α, F1A-α

Gene Name

FEM1B

Gene ID

10116

NCBI Gene ID

UniProt

Q9UK73

Accession Number

NP_056137

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide corresponding to aa 609- 622 of human F1A (accession no. AAF05314). This sequence is identical to that of mouse.

Target Antigen

Protein fem-1 homolog B

Target

F1Aa

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Substrate-rUniProtKB:Q9Z2G0, PubMed:10542291, PubMed:19330022, PubMed:24076122, PubMed:28118078, PubMed:29779948, PubMed:33398168, PubMed:33398170}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunohistochemistry: use at 5ug/mL. Positive control: Mouse liver tissue lysate. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat F1A (70kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Fas and tumor necrosis factor receptor 1 (TNFR1) are two members of the family of death receptors. A novel protein that associates with the intracellular domains of Fas and TNFR1 has been designated image (or FEM1 ). F1A is cleaved by CED-3 and caspase, and it associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1A induces apoptosis. F1A is expressed in a variety of human and mouse tissues.