Biotinylated Human VCAM-1 / CD106 Protein, Fc, Avitag™, premium grade

Vascular cell adhesion protein 1 (VCAM1) is also known as CD106, INCAM-100 and L1CAM, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. VCAM1 / CD106 contains 7 Ig-like C2-type (immunoglobulin-like) domains. CD106 / VCAM-1 is expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. L1CAM / VCAM-1 is Important in cell-cell recognition and appears to function in leukocyte-endothelial cell adhesion. CD106 / VCAM1 interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1 / VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. INCAM-100 / VCAM1 binds to ECMV-D capsid proteins and acts as a receptor for this virus.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 102.4 kDa. The protein migrates as 130 kDa±5 kDa under reducing (R) condition, and > 250 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

P19320-1

Expression Region

Phe 25 - Glu 698

Host

HEK293

Target

VCAM-1

Conjugation

Biotin-labeled

Tag

C-Fc & C-Avi

Source

Human

Stability

-20°C to -70°C for 24 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

95%

Bioactivity

Immobilized Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human VCAM-1, Fc,Avitag, premium grade (Cat. No. VC1-H82F6) with a linear range of 0.1-13 ng/mL (QC tested).