N-Ethylmaleimide-d5

N-Ethylmaleimide-d5 is the deuterium labeled N-Ethylmaleimide. N-Ethylmaleimide (NEM), a reagent that alkylates free sulfhydryl groups, is a cysteine protease inhibitor[1]. N-ethylmaleimide specific inhibits phosphate transport in mitochondria[2]. N-Ethylmaleimide is also a deubiquitinating enzyme inhibitor[3].

Product Specifications

CAS Number

[360768-37-2]

Product Name Alternative

NEM-d5

UNSPSC

12352005

Hazard Statement

H300, H311, H314, H317

Target

Cathepsin; Deubiquitinase

Type

Isotope-Labeled Compounds

Related Pathways

Cell Cycle/DNA Damage; Metabolic Enzyme/Protease

Field of Research

Others

Purity

99.90

Solubility

DMSO : 100 mg/mL (ultrasonic) |Ethanol : 12.5mg/mL (ultrasonic) |H2O : 50mg/mL (ultrasonic)

Smiles

O=C(C=C1)N(C([2H])([2H])C([2H])([2H])[2H])C1=O

Molecular Formula

C6H2D5NO2

Molecular Weight

130.16

Precautions

H300, H311, H314, H317

References & Citations

[1]Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53 (2) :211-216. |[2]Wu KH, et al. Cys32 and His105 are the critical residues for the calcium-dependent cysteine proteolyticactivity of CvaB, an ATP-binding cassette transporter. J Biol Chem. 2004 Jan 9;279 (2) :901-9.|[3]Hatase O , et al. Specific inhibition of phosphate transport in mitochondria by N-ethylmaleimide. Journal of Bioenergetics, 1973, 5 (1) :1-15.|[4]Choo YS, et al. Detection of protein ubiquitination. J Vis Exp. 2009; (30) :1293. Published 2009 Aug 19. doi:10.3791/1293