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Lipopolysaccharides, from E. coli O111:B4

Lipopolysaccharides, from E. coli O111:B4 (LPS, from Escherichia coli (O111:B4) ) are endotoxins and TLR4 activators extracted from Escherichia coli (E. coli O111:B4) and are classified as S (smooth) type LPS. Lipopolysaccharides (LPS), from E. coli O111:B4 possess the typical three-part structure: O-antigen, R3-type core oligosaccharide, and lipid A. Lipopolysaccharides (LPS), from E. coli O111:B4 activate TLR-4 in immune cells and can cause significant gastric diseases. Lipopolysaccharides (LPS), from E. coli O111:B4 can be used to induce cellular inflammation and establish animal models related to inflammation[1][2][3][4][5][6][7][8][9]. It is recommended to prepare a solution with concentration ≥2 mg/mL. Vortex thoroughly for more than 10 minutes. Due to the adsorption characteristics of LPS, silanized container or low adsorption centrifuge tubes should be used for aliquoting and storage, and mix thoroughly before use.

Product Specifications

Product Name Alternative

LPS, from Escherichia coli (O111:B4)

UNSPSC

12352200

Target

Toll-like Receptor (TLR)

Type

Biochemical Assay Reagents

Related Pathways

Immunology/Inflammation

Field of Research

Cancer; Infection

Assay Protocol

https://www.medchemexpress.com/lipopolysaccharides-from-e-coli-o111-b4.html

Solubility

H2O : 1 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

[Lipopolysaccharides,fromE.coliO111:B4]

References & Citations

[1]Kabanov DS, et al. Structural analysis of lipopolysaccharides from Gram-negative bacteria. Biochemistry (Mosc) . 2010 Apr;75 (4) :383-404.|[2]Ying Liu, et al. Podocyte-Released Migrasomes in Urine Serve as an Indicator for Early Podocyte Injury. Kidney Dis (Basel) . 2020 Nov;6 (6) :422-433.|[3]Heinrichs DE, et al. Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica. Mol Microbiol. 1998 Oct;30 (2) :221-32.|[4]Cai KC, et al. Age and sex differences in immune response following LPS treatment in mice. Brain Behav Immun. 2016 Nov;58:327-337.|[5]Vogel DY, et al. Human macrophage polarization in vitro: maturation and activation methods compared. Immunobiology. 2014 Sep;219 (9) :695-703|[6]Arioz BI, et al. Melatonin Attenuates LPS-Induced Acute Depressive-Like Behaviors and Microglial NLRP3 Inflammasome Activation Through the SIRT1/Nrf2 Pathway. Front Immunol. 2019 Jul 2;10:1511.|[7]Tezcan G, et al. Azithromycin and Ceftriaxone Differentially Activate NLRP3 in LPS Primed Cancer Cells. Int J Mol Sci. 2022 Aug 22;23 (16) :9484.|[8]Rialdi A, et al. Topoisomerase 1 inhibition suppresses inflammatory genes and protects from death by inflammation. Science. 2016 May 27;352 (6289) :aad7993.|[9]Mohamadi-Zarch SM, et al. Esculetin Alleviates Acute Liver Failure following Lipopolysaccharide/D-Galactosamine in Male C57BL/6 Mice. Iran J Med Sci. 2021 Sep;46 (5) :373-382.

Shipping Conditions

Room Temperature

Storage Conditions

-20°C, 3 years; 4°C, 2 years (Powder)

Scientific Category

Biochemical Assay Reagents

Clinical Information

No Development Reported

Available Sizes

Frequently Asked Questions

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