C12FDGlcU

C12FDGlcU is a lipophilic analog of fluorescein di-β-D-glucuronic acid. C12FDGlcU can be useful for the detection of β-glucuronidase (GUS) gene expression. C12FDGlcU can enter the cells and then be cleaved by β-glucuronidase, generating the yellow-colored, green-fluorescent fluorescein (Abs/Em of the reaction product: 495/518 nm) [1][2].

Product Specifications

CAS Number

423762-14-5

UNSPSC

12171500

Target

Fluorescent Dye; β-glucuronidase

Type

Dye Reagents

Related Pathways

Metabolic Enzyme/Protease; Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/c12fdglcu.html

Solubility

10 mM in DMSO

Smiles

O=C1OC2(C3=CC=C(NC(CCCCCCCCCCC)=O)C=C31)C4=CC=C(O[C@@H]5O[C@@H]([C@H]([C@@H]([C@H]5O)O)O)C(O)=O)C=C4OC6=CC(O[C@@H]7O[C@@H]([C@H]([C@@H]([C@H]7O)O)O)C(O)=O)=CC=C62

Molecular Formula

C44H51NO18

Molecular Weight

881.87

References & Citations

[1]A simple and general method for transferring genes into plants. Science. 1985 Mar 8;227 (4691) :1229-31.|[2]A J Fleming, et al. Fluorescent imaging of GUS activity and RT-PCR analysis of gene expression in the shoot apical meristem. Plant J. 1996 Oct;10 (4) :745-54.