Vaccinia Virus B18R Protein, His Tag

B18R is a type I interferon (IFN) -binding protein and it is encoded by the B18R open reading frame in the WR (Western Reserve) strain of vaccinia virus, which contains two Ig-like C2-type domains and one Ig-like V-type domain. B18R exists in a soluble and a membrane-bound form. As a type I IFN receptor, B18R counteracts the antiviral effects of host IFN-alpha/beta. Also, B18R acts as a soluble IFN-alpha receptor and thus inhibits the interaction between host IFN-alpha and its receptor.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 40.3 kDa. The protein migrates as 50-57 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P25213

Expression Region

His 20 - Glu 351

Host

HEK293

Target

B18R (Vaccinia Virus)

Conjugation

Unconjugated

Tag

C-10xHis

Source

Vaccinia Virus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized human IFN-α1a(106-01) at 2 μg/mL (100 μL/well) can bind Vaccinia Virus B18R, His Tag (Cat. No. B1R-H52H6) with a linear range of 3-13 ng/mL (QC tested).