Biotinyl Tyramide
Signal Amplifier
Product Specifications
Background
Biotinyl Tyramide is a biotin derivative that can be used as a reagent to amplify both IHC signals and in situ hybridization protocols (1). The secondary should be an HRP-labelled antibody or streptavidin conjugate; catalysis by the HRP results in the deposition of multiple tyramide molecules which can then be detected with a labeled streptavidin conjugate. Sensitivity has found to be 100 fold. It can be used with both chromogenic and fluorescent visualization methods (2).
CAS Number
41994-02-9
Product Name Alternative
(3aS,4S,6aR) -Hexahydro-N-[2- (4-hydroxyphenyl) ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide; Biotin-phenol
UNSPSC
41116105
Type
Amplifier
Source
Synthetic
Field of Research
Cell Signaling | Neuroscience
Purity
>98% (TLC) ; NMR (conforms)
Weight
0.005
Format
White powder
Solubility
May be dissolved in DMSO (35 mg/ml); or ethanol (20 mg/ml)
Molecular Formula
C18H25N3O3S
Molecular Weight
363.5
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Bobrow M.N. et al., (1989) J. Immunol. Methods. 125:279. 2. Kim S.H., et al. (2003) J Histochem Cytochem. 51(1): 129-132.
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