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Biotinyl Tyramide

Signal Amplifier

Product Specifications

Background

Biotinyl Tyramide is a biotin derivative that can be used as a reagent to amplify both IHC signals and in situ hybridization protocols (1). The secondary should be an HRP-labelled antibody or streptavidin conjugate; catalysis by the HRP results in the deposition of multiple tyramide molecules which can then be detected with a labeled streptavidin conjugate. Sensitivity has found to be 100 fold. It can be used with both chromogenic and fluorescent visualization methods (2).

CAS Number

41994-02-9

Product Name Alternative

(3aS,4S,6aR) -Hexahydro-N-[2- (4-hydroxyphenyl) ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide; Biotin-phenol

UNSPSC

41116105

Type

Amplifier

Source

Synthetic

Field of Research

Cell Signaling | Neuroscience

Purity

>98% (TLC) ; NMR (conforms)

Weight

0.005

Format

White powder

Solubility

May be dissolved in DMSO (35 mg/ml); or ethanol (20 mg/ml)

Molecular Formula

C18H25N3O3S

Molecular Weight

363.5

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Bobrow M.N. et al., (1989) J. Immunol. Methods. 125:279. 2. Kim S.H., et al. (2003) J Histochem Cytochem. 51(1): 129-132.

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