JC-1

JC-1 is a fluorescent dye that is able to pass across the mitochondrial membrane. It is used to measure mitochondrial membrane potential. At low membrane potentials, it remains a monomer and emits green-fluorescent light (λex=485 nm, λem=520 nm). At higher membrane potentials, it forms "J-aggregates", which emit red-fluorescent light (λex=520-570 nm, λem=570-610 nm). The ratio of red/green fluorescence is based only on the membrane potential, and not on mitochondrial size, shape or density.

5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide, , 5,6-dichloro-2-[ (E) -3- (5,6-dichloro-1,3-diethylbenzimidazol-3-ium-2-yl) prop-2-enylidene]-1,3-diethylbenzimidazole; iodide, Jc-1; 3520-43-2; JC-1 jodide; JC1 dye; CBIC2 (3) ; 5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide