Thapsigargin

Autophagy inhibitor

Product Specifications

Background

Thapsigargin is a cell-permeable inhibitor of the sarco-endoplasmatic reticulum Ca2+-ATPase (SERCA). This inhibition causes the release of intracellular Ca2+ stores. Thapsigargin also inhibits autophagy. This is due to the induction of and an ER stress response, which causes downstream effects that include an accumulation of autophagosomes in the cytosol. These intermediate structures never mature into amphisomes and autolysosomes, and the autophagy process cannot be completed.

Product Name Alternative

(3S,3aR,4S,6S,6AR,7S,8S,9bS)-6- (Acetyloxy)-2,3,3a,4,5,6,6a,7,8,9b-decahydro-3,3a-dihydroxy-3,6,9-trimethyl-8-[[(2Z)-2-methyl-1-oxo-2-butenyl]oxy]-2-oxo-4-(1-oxobutoxy)azuleno[4,5-b]furan-7-yl octanoate

UNSPSC

41116105

Source

Synthetic

Purity

>98% (TLC); NMR (Conforms)

Weight

0.005

Format

Colorless wax or white solid.

Solubility

Soluble in DMSO (65mg/mL); or Ethanol (20mg/mL)

Molecular Formula

C34H50O12

Molecular Weight

650.8

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Canová N.K., et al. (2007) Cell Biol Toxicol. 23(5): 337-54. 2. Ganley I.G., et al. (2011) Autophagy. 7(11): 1397-9. 3. Cheng Y., et al. (2013) Autophagy. 9(2): 208-19.