Tau (K18) P301L Mutant Monomers

Human Recombinant Tau (K18) P301L Mutant Monomers

Product Specifications

Background

Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1) . It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2) . Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3) . There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4) . K18 is a truncated form of human tau containing only the 4 microtubule binding repeats (5) . P301L (PL) is a mutation where proline is replaced by leucine at codon 301 of tau, and has been linked to frontotemporal dementia (6) .

Product Name Alternative

Tau monomer, Tau protein monomer, Tau protein, microtubule-associated protein Tau, MAPT, MAP, microtubule-associated protein, Tau-441, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 4, tubulin-associated unit

UNSPSC

12352202

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

4137

Swiss Prot

P10636

Cellular Locus

Expression System

E. coli

Host

E. coli

Origin Species

Human

Target

Tau (K18) P301L Mutant

Conjugation

No tag

Nature

Recombinant

Sequence

SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE

Applications

WB | SDS-PAGE | In vivo assay | In vitro assay

Field of Research

Purification Method

Ion-exchange Purified

Purification

Ion-exchange Purified

Limit Of Detection

Certified >95% pure using SDS-PAGE analysis. Low endotoxin <5 EU/mL @ 2mg/mL.

Concentration

2 mg/ml

Purity

>95%

Activity

Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when tau PFFs are combined with tau monomers.

Weight

0.05

Length

Partial

Buffer

10 mM HEPES, 100 mM NaCl pH 7.4

Molecular Weight

~15.1 kDa

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.

Additionnal Information

For corresponding PFFs, see catalog# SPR-330

References & Citations

1. www.alz.org/alzheimers-dementia/facts-figures 2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907) 3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497. 4. Goedert, M. and Spillantini, M. G. (2017). Mol Brain. 10:18. 5. Guo, J. and Lee, M.Y. (2013). FEBS Lett. 587(6): 717-723. 6. Alberici, A. et al. (2004). J Neurol Neurosurg Psychiatry. 75:1607-1610.

Shipping Conditions

Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.

Storage Conditions

-80ºC

Notes

For corresponding PFFs, see catalog# SPR-330

Protein Length

Partial

Background Reference 01

1. www.alz.org/alzheimers-dementia/facts-figures 2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907) 3. Matsumoto, G. et al. (2018) . Int J Mol Sci. 19, 1497. 4. Goedert, M. and Spillantini, M. G. (2017) . Mol Brain. 10:18. 5. Guo, J. and Lee, M.Y. (2013) . FEBS Lett. 587 (6) : 717-723. 6. Alberici, A. et al. (2004) . J Neurol Neurosurg Psychiatry. 75:1607-1610.

Location

AA Sequence

MSRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE