Rab5 Protein
Human Recombinant Rab5 Protein
Product Specifications
Background
Rab5 is a small GTPase of the Ras superfamily that plays a central role in regulating early endocytic trafficking. It cycles between an inactive GDP-bound state and an active GTP-bound state, a process tightly controlled by regulatory proteins such as GEFs, GAPs, and GDIs. In its active form, Rab5 associates with early endosomal membranes, where it recruits effector proteins like EEA1 and Rabaptin-5 to mediate vesicle docking, fusion, and cargo sorting. In neuroscience, Rab5 is essential for maintaining synaptic function and neuronal homeostasis through its regulation of receptor internalization, endosome maturation, and axonal transport. Dysregulation of Rab5 activity has been linked to impaired endosomal trafficking, a hallmark of several neurodegenerative diseases including Alzheimer’s and Parkinson’s. In Alzheimer’s disease, for example, Rab5 overactivation is associated with enlarged early endosomes and disrupted amyloid precursor protein (APP) processing, contributing to amyloid-beta accumulation. Rab5 also plays a role in neurotrophin signaling and synaptic plasticity, making it a key player in both neuronal development and degeneration. Its localization to early endosomes and the plasma membrane makes Rab5 a valuable marker for studying endocytic dysfunction in neurodegenerative models.
Product Name Alternative
Rab5, Rab5A, Ras-related protein Rab-5A, RAS associated protein RAB5A, RAB5A member RAS oncogene family
UNSPSC
12352202
UN Code
Non-hazardous
Hazard Statement
Non-hazardous
Gene ID
5868
Swiss Prot
P20339
Accession Number
BC001267
Cellular Locus
Cell Membrane | Early Endosome Membrane | Melanosome
Expression System
E. coli
Host
E. coli
Origin Species
Human
Target
Rab5
Conjugation
His tag
Nature
Recombinant
Sequence
MASRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN
Applications
WB | SDS-PAGE
Field of Research
Cancer | Heat Shock
Purification Method
Affinity Purified
Purification
Affinity Purified
Limit Of Detection
This product has been certified >90% pure using SDS-PAGE analysis.
Concentration
Lot/batch specific. See included datasheet.
Purity
>90%
Weight
0.05
Buffer
20mM Tris/HCl pH7.5, 0.45M NaCl, 10% glycerol, 0.5mM DTT
Molecular Weight
~26 kDa
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
References & Citations
1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7.
2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208.
3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412.
4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117.
5. Sonnichsen B., et al. (2000) J. Cell Biol. 149: 901-913
6. Woodman P.G. (2000) Traffic. 1: 695-701.
Shipping Conditions
Blue Ice or 4ºC
Storage Conditions
-20ºC
Background Reference 01
1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Sonnichsen B., et al. (2000) J. Cell Biol. 149: 901-913 6. Woodman P.G. (2000) Traffic. 1: 695-701.
Location
Cell Membrane | Early Endosome Membrane | Melanosome
AA Sequence
MASRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN
Immunogen Species
Human
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