GRP78 (Bip) Protein

Human Recombinant GRP78 (Bip) Protein

Product Specifications

Background

GRP78, also known as BiP (Binding Immunoglobulin Protein), is a key endoplasmic reticulum (ER) chaperone involved in protein folding, quality control, and the unfolded protein response (UPR) . It is a member of the HSP70 family and is upregulated during ER stress to maintain cellular homeostasis. In neuroscience, GRP78 plays a protective role against ER stress-induced apoptosis, a common feature in neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and ALS. Neurons are particularly sensitive to disruptions in protein folding, and GRP78 helps mitigate this by stabilizing misfolded proteins and regulating UPR signaling pathways. Reduced GRP78 expression has been observed in the brains of Alzheimer’s patients, correlating with increased protein aggregation and neuronal loss. Conversely, upregulation of GRP78 has been shown to confer neuroprotection in models of glutamate toxicity and oxidative stress. GRP78 also influences neurodevelopment and synaptic plasticity, making it a critical factor in both disease and normal brain function. Its role in modulating ER stress responses positions it as a potential biomarker and therapeutic target for neurodegenerative and neuroinflammatory disorders.

Product Name Alternative

BiP, GRP78, HSPA5, MIF2, Immunoglobulin heavy chain binding

UNSPSC

12352202

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

3309

Swiss Prot

P11021

Accession Number

NM_005347

Cellular Locus

Endoplasmic Reticulum Lumen | Melanosome

Expression System

E. coli

Host

E. coli

Origin Species

Human

Target

GRP78 (Bip)

Conjugation

His tag

Nature

Recombinant

Sequence

MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL

Applications

WB | SDS-PAGE | ATPase Activity Assay | Functional Assay | ELISA

Field of Research

Purification Method

Affinity Purified

Purification

Affinity Purified

Limit Of Detection

This product has been certified >90% pure using SDS-PAGE analysis. The protein shows ATPase activity using a Malachite Green assay.

Concentration

Lot/batch specific. See included datasheet.

Purity

>90%

Activity

ATPase active

Weight

0.05

Buffer

50mM Tris/HCl, pH 7.5, 150mM NaCl, 10% glycerol

Molecular Weight

~78 kDa

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.

References & Citations

1. Cho S., et al (2007) Mol Cell Biol. 27(1): 368-83. 2. Yang Y., et al. (1998) J Biol Chem. 273: 25552-25555. 3. Luo S., et al (2006) 26(15): 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19: 4333-4336. 6. Laquerre S., et al. (1998) J. Virology. 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res. 65(13): 5785-91.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Background Reference 01

1. Cho S., et al (2007) Mol Cell Biol. 27 (1) : 368-83. 2. Yang Y., et al. (1998) J Biol Chem. 273: 25552-25555. 3. Luo S., et al (2006) 26 (15) : 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19: 4333-4336. 6. Laquerre S., et al. (1998) J. Virology. 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res. 65 (13) : 5785-91.