HSC70 Protein

Human Recombinant HSC70 Protein

Product Specifications

Background

HSC70 (Heat Shock Cognate 70) is a constitutively expressed member of the HSP70 family, functioning as a molecular chaperone involved in protein folding, trafficking, and degradation. Unlike its inducible counterpart HSP70, HSC70 is present under normal physiological conditions and plays a continuous role in maintaining proteostasis. In the nervous system, HSC70 is essential for synaptic function, axonal transport, and the clearance of misfolded proteins. It is particularly relevant in neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s, where protein aggregation and impaired degradation pathways are central to disease progression. HSC70 binds to nascent and partially unfolded proteins, preventing aggregation and facilitating proper folding. It also participates in chaperone-mediated autophagy (CMA), a selective degradation pathway critical for removing damaged or misfolded proteins in neurons. Dysregulation of HSC70 or CMA has been linked to the accumulation of neurotoxic proteins and neuronal dysfunction. Its ATP-dependent conformational changes and interactions with co-chaperones enable HSC70 to adapt to cellular stress and support recovery. As a result, HSC70 is a promising target for therapeutic strategies aimed at enhancing protein clearance and restoring neuronal health in neurodegenerative conditions.

Product Name Alternative

Constitutive heat shock 70, HSC54, HSC71, HSC73, HSP71, HSP74, HSPA10, HSPA8, LAP1, NIP71

UNSPSC

12352202

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

3312

Swiss Prot

P11142

Accession Number

AF352832

Cellular Locus

Cytoplasm | Melanosome

Expression System

E. coli

Host

E. coli

Origin Species

Human

Target

HSC70

Conjugation

His tag

Nature

Recombinant

Applications

WB | SDS-PAGE | ATPase Activity Assay | Functional Assay | ELISA

Field of Research

Cancer | Heat Shock

Purification Method

Affinity Purified

Purification

Affinity Purified

Limit Of Detection

This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 3.2µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay.

Concentration

Lot/batch specific. See included datasheet.

Purity

>90%

Activity

ATPase active

Weight

0.1

Buffer

20mM Tris/HCl pH7.5, 1mM EDTA

Molecular Weight

~70 kDa

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.

References & Citations

1. Brown C. L., et al. (1993) J.Cell Biol. 120(5): 1101-1112 2. Boorstein W. R., Ziegelhoffer T. & Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell. 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294. 6. Brown C. L., et al. (1996) J. Biol. Chem. 271(2): 833-840.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Background Reference 01

1. Brown C. L., et al. (1993) J.Cell Biol. 120 (5) : 1101-1112 2. Boorstein W. R., Ziegelhoffer T. & Craig E.A. (1993) J. Mol. Evol. 38 (1) : 1-17. 3. Rothman J. (1989) Cell. 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294. 6. Brown C. L., et al. (1996) J. Biol. Chem. 271 (2) : 833-840.