WNK1 Antibody (pSer382)

WNK1 (With No Lysine [K] Kinase 1), particularly in its phosphorylated form at serine 382 (pSer382), plays a central role in regulating neuronal ion transport and cellular volume—processes that are critically disrupted in neurodegenerative diseases. As a master kinase, WNK1 activates downstream effectors such as SPAK and OSR1, which control the activity of cation-chloride cotransporters like NKCC1 and KCC2. These transporters are essential for maintaining chloride balance, synaptic inhibition, and overall neuronal excitability. In the brain, WNK1 (pSer382) responds to osmotic stress and changes in intracellular chloride levels, initiating signaling pathways that influence neuron survival, glial cell function, and cerebrospinal fluid regulation. Dysregulation of this pathway has been associated with brain edema, ischemic injury, and chronic neuroinflammation—key features of disorders such as Alzheimer’s disease, Parkinson’s disease, and epilepsy. Targeting WNK1 phosphorylation, especially at Ser382, is emerging as a promising therapeutic strategy. By restoring ionic equilibrium and modulating neuroinflammatory responses, WNK1 (pSer382) offers a novel entry point for interventions aimed at slowing or preventing neurodegeneration. As research progresses, WNK1 (pSer382) is gaining recognition as a critical molecular link between ion homeostasis and brain health, with broad implications for neuroscience and neurotherapeutics.

WNK1, WNK Lysine Deficient Protein Kinase 1, PRKWNK1, PPP1R167, HSN2, HSAN2, Protein Kinase With No Lysine 1, Serine/Threonine-Protein Kinase WNK1, Protein Kinase Lysine-Deficient 1, Kinase Deficient Protein, KDP, P65, PSK, Prostate-Derived Sterile 20-Like Kinase, Erythrocyte 65 kDa Protein, KIAA0344, HWNK1, EC 2.7.11.1, MGC163339, MGC163341

Highly expressed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.

A 1:250 dilution of SPC-1097 was sufficient for detection of WNK1 (pSer382) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.