HSP70 Antibody (Acetyl Lys77) : Biotin

Rabbit Anti-Human HSP70 Acetyl Lys77 Polyclonal

Product Specifications

Background

HSP70 proteins are a highly conserved family of 70-kDa molecular chaperones that play a central role in protein folding, stabilization, and transport across nearly all cellular compartments—including the cytosol, nucleus, mitochondria, endoplasmic reticulum, and chloroplasts. These proteins are encoded by a multigene family and are upregulated in response to cellular stress, making them key regulators of proteostasis. The HSP70 Acetylated Lysine 77 Antibody is a powerful tool for detecting HSP70 proteins specifically modified at lysine 77, a post-translational modification (PTM) that may influence chaperone activity, protein-protein interactions, and cellular localization. Acetylation at this site occurs within the highly conserved N-terminal ATP-binding domain, which is essential for HSP70’s conformational cycling and substrate release. This antibody may help researchers to monitor acetylation-specific regulation of HSP70 under stress conditions; investigate the role of lysine 77 acetylation in neurodegenerative diseases, cancer, and aging; or differentiate between modified and unmodified HSP70.

CAS Number

9007-83-4

Specifications

Binds to acetylated lysine 77 on HSP70. Detects 70 kDa band.

Product Name Alternative

HSPA1A, HSPA1B, HSPA1, HSP70, HSP70-1, HSP70.1, HSP70-2, HSP72, HSP73, HSX70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B, HSP70 Acetylated lysine 77, HSP70 Acetyl Lys77

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

3303

Swiss Prot

P0DMV8/P0DMV9

Accession Number

NP_005336.3

Cellular Locus

Cytoplasm | Nucleus

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide from the N-terminal of Human HSP70 Acetyl Lys77 (1-100 aa), conjugated to Keyhole Limpet Haemocyanin (KLH) .

Target

HSP70 Acetyl Lys77

Clonality

Polyclonal

Conjugation

Biotin

Type

Polyclonal

Applications

WB | ELISA | ICC/IF | IHC

Validated Applications

WB,IHC,ICC/IF |ELISA

Field of Research

Purification

Peptide Affinity Purified

Detection Range

A 1:1000 dilution of SPC-743 was sufficient for detection of HSP70 Acetyl Lys77 in 10 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Concentration

0.5mg/mL

Dilution

WB (1:1000) ; ELISA (1:1000) ; ICC/IF (1:60) ; IHC (1:50) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

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