HSP70 Antibody (Acetyl Lys77)

Rabbit Anti-Human HSP70 Acetyl Lys77 Polyclonal

Product Specifications

Background

HSP70 proteins are a highly conserved family of 70-kDa molecular chaperones that play a central role in protein folding, stabilization, and transport across nearly all cellular compartments—including the cytosol, nucleus, mitochondria, endoplasmic reticulum, and chloroplasts. These proteins are encoded by a multigene family and are upregulated in response to cellular stress, making them key regulators of proteostasis. The HSP70 Acetylated Lysine 77 Antibody is a powerful tool for detecting HSP70 proteins specifically modified at lysine 77, a post-translational modification (PTM) that may influence chaperone activity, protein-protein interactions, and cellular localization. Acetylation at this site occurs within the highly conserved N-terminal ATP-binding domain, which is essential for HSP70’s conformational cycling and substrate release. This antibody may help researchers to monitor acetylation-specific regulation of HSP70 under stress conditions; investigate the role of lysine 77 acetylation in neurodegenerative diseases, cancer, and aging; or differentiate between modified and unmodified HSP70.

CAS Number

9007-83-4

Specifications

Binds to acetylated lysine 77 on HSP70. Detects 70 kDa band.

Product Name Alternative

HSPA1A, HSPA1B, HSPA1, HSP70, HSP70-1, HSP70.1, HSP70-2, HSP72, HSP73, HSX70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B, HSP70 Acetylated lysine 77, HSP70 Acetyl Lys77

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

3303

Swiss Prot

P0DMV8/P0DMV9

Accession Number

NP_005336.3

Cellular Locus

Cytoplasm | Nucleus

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide from the N-terminal of Human HSP70 Acetyl Lys77 (1-100 aa), conjugated to Keyhole Limpet Haemocyanin (KLH) .

Target

HSP70 Acetyl Lys77

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal

Applications

WB | ELISA | ICC/IF | IHC

Validated Applications

WB,ELISA,ICC/IF,IHC

Field of Research

Purification

Peptide Affinity Purified

Detection Range

A 1:1000 dilution of SPC-743 was sufficient for detection of HSP70 Acetyl Lys77 in 10 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Concentration

0.5mg/mL

Dilution

WB (1:1000) ; ELISA (1:1000) ; ICC/IF (1:60) ; IHC (1:50) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

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