Alpha Synuclein Antibody (pSer129)

Alpha-synuclein (SNCA) is a neuronal protein predominantly expressed in the brain, where it is enriched at presynaptic terminals and plays a key role in synaptic function (1) . It is also highly expressed in mitochondria-rich regions such as the olfactory bulb, hippocampus, striatum, and thalamus, suggesting a role in mitochondrial dynamics and neuronal energy metabolism (2) . Functionally, alpha-synuclein interacts with tubulin (3), indicating a potential role as a microtubule-associated protein involved in cytoskeletal organization and intracellular transport. It is essential for cognitive development, with SNCA inactivation linked to impaired spatial learning and working memory (4) . Pathologically, SNCA aggregates are a major non-Aβ component of amyloid plaques in Alzheimer’s disease and a defining feature of Lewy body inclusions in Parkinson’s disease (PD) . PD is characterized by the progressive accumulation of alpha-synuclein and ubiquitin-positive inclusions in vulnerable neurons, contributing to neurodegeneration (5, 6) . Critically, phosphorylation of alpha-synuclein at serine 129 (pSer129) is a key post-translational modification associated with disease pathology. Approximately 90% of alpha-synuclein found in Lewy bodies is phosphorylated at Ser129, compared to only a small fraction in the healthy brain (7, 8) . This modification is widely used as a biomarker for pathological alpha-synuclein and is a focal point in therapeutic and diagnostic research targeting synucleinopathies.

Binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein. Detects bands at 100, 75, 45, 15 kDa. Bands above 15 kDa are oligomers.

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Neuroscience | Neurodegeneration | Alzheimer's Disease | Tangles & Tau | Neuroscience | Neurodegeneration | Parkinson's Disease | Synuclein | Neuroscience | Neurodegeneration | Multiple System Atrophy

A 1:1000 dilution of SPC-742 was sufficient for detection of Alpha Synuclein pSer129 in 15 µg of human brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013) . 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277 (3) : 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231 (1) : 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388 (6645) : 839-840. 6. Mezey E., et al. (1998) Nat Med. 4 (7) : 755-757. 7. Fujiwara H., et al. (2002) Nat Cell Biol. 4 (2) :160-4. 8. Anderson J.P., et al. (2006) J Biol Chem. 281 (40) :29739-52.

Binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein. Detects bands at 100, 75, 45, 15 kDa. Bands above 15 kDa are oligomers.