DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-FLAG® M2 Antibody) : FITC

The DYKDDDDK tag, also known as the FLAG® tag, is a widely used epitope tag in molecular biology and protein research. Comprising eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), this hydrophilic sequence is engineered into recombinant proteins to facilitate detection, purification, and localization studies. Its small size minimizes interference with protein function, making it ideal for fusion protein applications in both prokaryotic and eukaryotic systems. Anti-DYKDDDDK tag antibodies, such as those equivalent to Sigma’s Anti-FLAG® M2, are monoclonal or polyclonal antibodies specifically designed to recognize the DYKDDDDK epitope with high affinity and specificity. These antibodies are essential tools in Western blotting, immunoprecipitation, immunofluorescence, and ELISA, enabling researchers to track tagged proteins with precision. The FLAG tag system is particularly valued for its compatibility with gentle elution conditions, preserving protein structure and function during purification. Additionally, the tag’s unique sequence ensures minimal cross-reactivity with endogenous proteins, enhancing signal clarity in complex biological samples. Anti-DYKDDDDK antibodies are validated across multiple species and applications, making them a cornerstone in proteomics, structural biology, and therapeutic protein development. Their robust performance and commercial availability have established them as a gold standard in epitope tagging technologies.

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A 1:1000 dilution of SPC-720 was sufficient for detection of DYKDDDDK Tag in 15 µg of DYKDDDDK (FLAG) Positive Control Lysate cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.