Human HMGB1 Protein, Mouse IgG2a Fc Tag, low endotoxin
Human HMGB1 Protein, Mouse IgG2a Fc Tag (HM1-H5255) is expressed from human 293 cells (HEK293) . It contains AA Gly 2 - Glu 215 (Accession # P09429-1) .
Product Specifications
Background
High-mobility group protein B1 (HMGB1) is also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release. This positions HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.
Specifications
This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 51.6 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P09429-1
Expression Region
Gly 2 - Glu 215
Host
HEK293
Target
HMGB1
Conjugation
Unconjugated
Tag
N-mFc (mIgG2a)
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
0.1 EU per μg
Purity
95%
Format
Powder
Buffer
50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
Additives
Trehalose
Molecular Weight
51.6 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
500ug*2
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/HM1-H5255.pdf
Species
Human
Curated Selection
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