Choline Acetyltransferase Antibody

Rabbit Anti-Human Choline Acetyltransferase Polyclonal

Product Specifications

Background

Choline acetyltransferase (ChAT) catalyzes the synthesis of acetylcholine (ACh), a neurotransmitter essential for motor control, memory, and autonomic function. ChAT is selectively expressed in cholinergic neurons, making it a reliable marker for cholinergic system integrity. Loss of ChAT activity is a hallmark of several neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and Down syndrome. Reduced ChAT expression correlates with cognitive decline and impaired synaptic transmission in affected brain regions. As a neuron-specific enzyme with a direct role in neurotransmitter synthesis, ChAT is both a diagnostic marker and a therapeutic target in disorders characterized by cholinergic dysfunction.

CAS Number

9007-83-4

Specifications

Predicted molecular weight at ~82.5kDa. Observed molecular weights between 68-70kDa.

Product Name Alternative

CHAT, Choline acetyltransferase, Acetyl-CoA choline O-acetyltransferase, Choline acetylase, CLAT_HUMAN, CMS1A

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

1103

Swiss Prot

P28329

Accession Number

NP_001136401.1

Cellular Locus

Cytoplasm | Cytosol | Mitochondrion | Nucleus

Host

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase

Target

Choline Acetyltransferase

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal

Applications

WB | ICC/IF

Validated Applications

WB,ICC/IF

Field of Research

Purification

Peptide Affinity Purified

Detection Range

A 1:1000 dilution of SPC-706 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Houser C.R., Crawford G.D., Barber R.P., Salvaterra P.M., Vaughn J.E. (1983) Brain Research. 266 (1) : 97-119. 2. Karson C.N., Casanova M.F., Kleinman J.E.m Griffin W.S. (1993) Am J Psychiatry. 150: 454-459. 3. Baskins D.S., et al. (1999) Arch Neurol. 56: 1121-1123.