P53 Antibody: APC

Rabbit Anti-Human p53 Polyclona

Product Specifications

Background

P53 (tumor protein 53) is a transcription factor best known for its role in tumor suppression, but it also plays a critical role in neuronal survival, DNA repair, and apoptosis. Often referred to as the “guardian of the genome, ” p53 maintains genomic stability by regulating cell cycle checkpoints and initiating cell death in response to DNA damage. In the brain, p53 is activated by oxidative stress, excitotoxicity, and mitochondrial dysfunction—key features of neurodegenerative diseases. Overactivation of p53 can lead to excessive neuronal apoptosis, contributing to the progression of Alzheimer’s, Parkinson’s, and Huntington’s disease. Targeting p53 signaling pathways offers a promising strategy to modulate neuronal death and enhance neuroprotection in degenerative conditions.

CAS Number

9007-83-4

Specifications

Predicted molecular weight at ~43.7kDa. Observed bands at ~53kDa.

Product Name Alternative

TP53, Tumor Protein 53, BCC7, LFS1, TRP53, p53 tumor suppressor

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

7157

Swiss Prot

P04637

Accession Number

NP_000537.3

Cellular Locus

Cytoplasm | Nucleus | PML body | Endoplasmic Reticulum | Mitochondrion matrix

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide from the C-terminal of human tumor protein p53.

Target

p53

Clonality

Polyclonal

Conjugation

APC

Type

Polyclonal

Applications

WB | ICC/IF

Validated Applications

IHC,ICC/IF

Field of Research

Purification

Peptide Affinity Purified

Detection Range

A 1:1000 dilution of SPC-682 was sufficient for detection of p53 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Georescu C.V., Saftoiu A., Georgescu C.C., Ciurea R. and Ciurea T. (2007) J Gastrointestin Liver Dis. 16 (2) : 133-9. 2. Strachan T., et al. (1999) Human Molecular Genetics 2. Ch. 18: Cancer Genetics. 3. Hansen J.E., et al. (2007) Cancer Research 67: 1769-1774. 4. Di Cesare E., et al. (2001) Ann Clin Lab Sci 31: 253-8. 5. Akere A., Otegbayo J.A. (2007) Singapore Med J. 48 (1) : 41-4.