WDR45 Antibody

WDR45, also known as WIPI-4, is a 360-amino acid protein containing WD40 repeats, which are structural motifs involved in protein-protein interactions. As a member of the WIPI (WD repeat domain phosphoinositide-interacting) protein family, WDR45 plays a critical role in autophagy—a cellular degradation and recycling process essential for neuronal health and homeostasis. WDR45 exists in three alternatively spliced isoforms and is ubiquitously expressed, with highest levels in heart and skeletal muscle. In the nervous system, WDR45 is vital for maintaining cellular quality control, particularly in long-lived neurons that are highly susceptible to proteotoxic stress. Dysfunction in WDR45-mediated autophagy has been directly linked to neurodegeneration with brain iron accumulation (NBIA), specifically types 4 and 5, which are characterized by progressive cognitive decline, motor dysfunction, and iron deposition in the basal ganglia. Mutations in the WDR45 gene disrupt autophagosome formation and trafficking, leading to the accumulation of damaged proteins and organelles—a hallmark of many neurodegenerative diseases, including Parkinson’s and Alzheimer’s. As such, WDR45 is increasingly recognized as a potential biomarker and therapeutic target in neurodegenerative disease research. Understanding the molecular mechanisms of WDR45 offers valuable insights into the intersection of autophagy, iron metabolism, and neuronal survival.

WDR45, WD Repeat Domain 45, WIPI4, WIPI-4, WD Repeat Domain Phosphoinositide-Interacting Protein 4, WD Repeat-Containing Protein 45, WD Repeat Domain X-Linked 1, WDRX1, WDRXI4, JM5, NBIA4, NBIA5, WD45 Repeat Protein Interacting With Phosphoinositides, Progranulin-Associated Neurodegeneration Protein, MGC163339, MGC163341

Ubiquitously expressed, with high expression in skeletal muscle and heart. Weakly expressed in liver and placenta. Expression is down-regulated in pancreatic and in kidney tumors.

A 1:1000 dilution of SPC-651 was sufficient for detection of WIPI4 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

1. http://www.genecards.org/cgi-bin/carddisp.pl?gene=WDR45 2. Strausberg R.L., et al. (2002) Proc Natl Acad Sci U S A. 99 (26) : 16899–16903. 3. Haack T.B., et al. (2012) Am J Hum Genet. 91 (6) : 1144–1149.