ATG4B Antibody: ATTO 390

Rabbit Anti-Human ATG4B Polyclonal

Product Specifications

Background

Autophagy related 4B, cysteine peptidase (ATG4B or APG4B) is a C-54 cysteine protease that also belongs to the family of autophagin poteins. Specifically it cleaves LC3, and mutations can be associated with strong inhibition of autophagosome formation.

CAS Number

9007-83-4

Specifications

Detects ~44 kDa.

Product Name Alternative

Cancer, Autophagy, Cardiovascular System, Heart, Cell Signaling

UNSPSC

12352203

Gene ID

23192

Swiss Prot

Q9Y4P1

Accession Number

NP_037457.3

Cellular Locus

Cytoplasm

Host

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Synthetic peptide from the N-terminal to mid of Human ATG4B (aa. 94-106)

Target

ATG4B

Clonality

Polyclonal

Conjugation

ATTO 390

Tissue Specificity

Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

Validated Applications

IHC,ICC/IF

Purification

Peptide Affinity Purified

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36mM Ethanolamine, and 9.09mM Sodium Bicarbonate in 90.9% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Kabeya Y., et al. (2004) J Cell Sci. 117: 2805-2812. 2. Tanida I., et al. (2004) J Biol Chem. 279: 36268-36276. 3. Hemelaar J., et al. (2003) J Biol Chem. 278: 51841-51850.