Beclin 1 Antibody

Rabbit Anti-Human Beclin 1 Polyclonal

Product Specifications

Background

Beclin 1, the mammalian ortholog of yeast Atg6, is a key regulator of autophagy—a cellular degradation pathway essential for maintaining neuronal homeostasis. As a core component of the class III phosphatidylinositol 3-kinase (PI3K) complex, Beclin 1 orchestrates autophagosome formation and maturation, processes critical for the clearance of damaged organelles and misfolded proteins. In the nervous system, Beclin 1 plays a pivotal role in neurodevelopment and neuroprotection. Dysregulation of Beclin 1-mediated autophagy has been implicated in a range of neurodegenerative diseases, including Alzheimer’s, Parkinson’s, and Huntington’s disease, where impaired protein clearance contributes to synaptic dysfunction and neuronal loss. Beclin 1 is primarily localized to cytoplasmic structures, including mitochondria, but overexpression studies have also revealed nuclear localization, suggesting additional regulatory roles. Notably, reduced Beclin 1 expression has been observed in the hippocampus of individuals with schizophrenia, linking autophagy deficits to psychiatric disorders. Furthermore, Beclin 1 may contribute to neuroimmune defense. It has been shown to protect against infection by neurovirulent strains of Sindbis virus, highlighting its role in antiviral responses within the central nervous system. Given its multifaceted functions in autophagy, neurodevelopment, and immune defense, Beclin 1 is a promising target for therapeutic strategies aimed at restoring cellular homeostasis in neurodegenerative and neuropsychiatric disorders.

CAS Number

9007-83-4

Specifications

Predicted molecular weight at ~51kDa. Observed a secondary band on some lysates at ~30kDa.

Product Name Alternative

Beclin 1, BECN1, BECN1_HUMAN, APG6, BCL-2 interacting protein beclin, Beclin 1 autophagy related, GT197, VPS30

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

8678

Swiss Prot

Q14457

Accession Number

NP_001300927.1

Cellular Locus

Host

Rabbit

Species Reactivity

Human,Rat

Immunogen

Synthetic peptide from the mid-protein of human Beclin 1

Target

Beclin 1

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal

Applications

WB | ICC/IF

Validated Applications

WB,ICC/IF

Field of Research

Purification

Peptide Affinity Purified

Detection Range

A 1:1000 dilution of SPC-600 was sufficient for detection of Beclin1 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Zhong Y., et al. (2009) Nat Cell Biol. 11 (4) : 468-476. 2. Liang X.H., et al. (2001) Cancer Res. 61: 3443-3449. 3. Merenlender-Wagner A., et al. (2013) Mol. Psychiatry 20:126-132. 4. Liang X.H., et al. (1998) J Virol. 72: 8586-8596.