Amyloid Oligomers (A11) Antibody: RPE

Amyloid oligomers are soluble, misfolded protein assemblies that represent a critical early stage in the formation of amyloid fibrils. Detected by the conformation-specific A11 antibody, these oligomeric species are increasingly recognized as the most neurotoxic form of amyloid aggregates in neurodegenerative diseases. Unlike mature fibrils, amyloid oligomers disrupt cellular function through membrane permeabilization, oxidative stress, and synaptic impairment. Oligomerization can occur even in non-disease-related proteins under conditions of partial misfolding or denaturation, highlighting the intrinsic amyloidogenic potential of many polypeptides. In pathological contexts, amyloid oligomers are central to the progression of disorders such as Alzheimer’s disease (via amyloid-β and tau), Parkinson’s disease (via α-synuclein), and Huntington’s disease (via polyglutamine-expanded huntingtin) . These toxic intermediates precede the formation of insoluble plaques and tangles, making them valuable biomarkers and therapeutic targets. The A11 antibody has become a powerful tool in neuroscience research, enabling the selective detection of prefibrillar oligomers across diverse amyloidogenic proteins. Its application has advanced our understanding of early aggregation events and their role in synaptic dysfunction and neuronal loss. As the field shifts toward early intervention strategies, amyloid oligomers—and their detection via A11—are at the forefront of neurodegenerative disease research, offering critical insights into disease mechanisms and potential avenues for therapeutic development.

Recognizes all types of amyloid oligomers. Appears to recognize a peptide backbone epitope that is common to amyloid oligomers, but is not found in native proteins, amyloidogenic monomer or mature amyloid fibrils.

Amyloid Oligomer alpha beta, A11, Amyloid Oligomer AlphaBeta, APP, Amyloid beta A4 protein, ABPP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, A4, AD1

A 1:200 dilution of SPC-506 was sufficient for detection of amyloid oligomers in 10 µg of mouse brain lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

The suggested dilution for the current lot of SPC-506 in Western Blot and Dot Blot is 1:100; optimal dilutions for assays should be determined by the user.

1. Glabe C.G. (2004) Trends Biochem Sci. 29 (10) : 542-547. 2. Kayed R., et al. (2004) J Bio. Chem. 279: 46363-46366. 3. Kayed R., et al. (2003) Science. 300 (5618) : 486-489.

Recognizes all types of amyloid oligomers. Appears to recognize a peptide backbone epitope that is common to amyloid oligomers, but is not found in native proteins, amyloidogenic monomer or mature amyloid fibrils.