Aquaporin 4 Antibody

Rabbit Anti-Rat Aquaporin 4 Polyclonal

Product Specifications

Background

Aquaporin 4 (AQP4) is a key water channel protein predominantly expressed in the central nervous system, where it plays a critical role in maintaining brain water balance. While it is also found in the basolateral membrane of renal collecting duct cells, its most significant physiological and pathological functions are observed in the brain, particularly in astrocyte endfeet at the blood-brain barrier and around brain ventricles. AQP4 facilitates bidirectional water transport in response to osmotic gradients, contributing to cerebrospinal fluid dynamics, neurovascular coupling, and the clearance of metabolic waste via the glymphatic system. Its expression is rapidly upregulated in response to central nervous system (CNS) injury, including trauma, ischemia, and neuroinflammation, making it a key player in the development of cerebral edema and gliosis. Importantly, AQP4 is the primary autoimmune target in neuromyelitis optica spectrum disorder (NMOSD), a severe inflammatory demyelinating disease of the CNS. Autoantibodies against AQP4 (AQP4-IgG) lead to astrocyte damage, blood-brain barrier disruption, and secondary neuronal injury. Beyond NMOSD, dysregulation of AQP4 has been implicated in Alzheimer’s disease, Parkinson’s disease, and multiple sclerosis, where impaired water clearance and neuroinflammation contribute to disease progression. As such, AQP4 is increasingly recognized as a promising biomarker and therapeutic target in neurodegenerative disease research.

CAS Number

9007-83-4

Specifications

Detects ~35kDa. May detect larger glycosylated bands ~50kDa.

Product Name Alternative

Aquaporin 4, Aquaporin-4, AQP4, AQP-4, AQP 4, AQP4_HUMAN, Aquaporin type 4, Aquaporin4, HMIWC 2, HMIWC2, Mercurial insensitive water channel, Mercurial-insensitive water channel, MGC22454, MIWC, WCH 4, WCH4

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

25293

Swiss Prot

P47863

Accession Number

NP_036957.1

Cellular Locus

Membrane

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Produced against the C-terminal peptide (Sequence N-CTKGSYMEVEDNRSQVETED) of rat aquaporin 4

Target

Aquaporin 4

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal

Applications

WB | IHC | ICC/IF

Validated Applications

WB,IHC,ICC/IF

Field of Research

Neuroscience | Neurotransmission | Ion Channels | Aquaporins

Purification

Protein A Purified

Detection Range

0.5 µg/ml of SPC-505 was sufficient for detection of aquaporin 4 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:2000), IHC (1:200), ICC/IF (1:400) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39 (4) : 361-396. 2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91 (14) : 6255-6258. 3. Agre P., Nielsen S. (1996) Nephrologie. 17 (7) : 409-415. 4. Nagelhus E.A., Mathiisen T.M., Ottersen O.P. (2004) Neuroscience. 129 (4) : 905-913. 5. Lennon V.A., Kryzer T.J., Pittock S.J., Verkman A.S., Hinson S.R. (2005) J Exp Med. 202 (4) : 473-477. 6. Bradl M., Lassmann D.H. (2008) Int. MS Journal/MS Forum. 15 (3) : 75-78.