NKCC2 Antibody: RPE

Rabbit Anti-Rat NKCC2 Polyclonal

Product Specifications

Background

NKCC2 (Na⁺-K⁺-2Cl⁻ cotransporter 2), encoded by SLC12A1, is a renal-specific transporter located in the thick ascending limb of the loop of Henle. Although classically associated with renal sodium reabsorption and targeted by loop diuretics, NKCC2’s role in the brain is gaining attention. Emerging studies suggest that NKCC2 may influence cerebrospinal fluid composition, neuronal excitability, and osmoregulation. Dysregulation of NKCC2 could contribute to altered ion gradients and excitotoxicity, processes implicated in neurodegenerative diseases such as Alzheimer’s and epilepsy. Understanding NKCC2’s extrarenal functions may uncover novel mechanisms of ion homeostasis and therapeutic opportunities in neurodegeneration.

CAS Number

9007-83-4

Specifications

Detects ~160kDa.

Product Name Alternative

SLC12A1, sodium potassium chloride cotransporter 2, sodium-potassium-chloride cotransporter isoform 2, BSC1, MBSC1, Na-K-2Cl cotransporter, NKCC2A variant A, RBSC

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

25065

Swiss Prot

Q5PQV1

Accession Number

NP_001257546.1

Cellular Locus

Membrane

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody designation L224) .

Target

NKCC2

Clonality

Polyclonal

Conjugation

RPE

Type

Polyclonal

Applications

WB | IHC

Validated Applications

WB,IHC,ICC/IF

Field of Research

Cancer | Neuroscience | Pumps/Transporters | Ion Pumps/Transporters

Purification

Protein A Purified

Detection Range

1 µg/ml of SPC-401 was sufficient for detection of NKCC2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; IHC (1:25) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Caceres P.S., Ares G.R., and Ortiz P.A. (2009) J Biol Chem. 284 (37) : 24965-24971. 2. Xue H., et al. (2009) Neurogastroentrol Motil. 21 (10) : 1068-1089.