NHE3 Antibody: RPE
Rabbit Anti-Rat NHE3 Polyclonal
Product Specifications
Background
Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).
Specifications
Detects ~84kDa.
Product Name Alternative
Cell Signaling, Neuroscience, Pumps/Transporters, Ion Pumps/Transporters, Cancer
UNSPSC
12352203
Gene ID
24784
Swiss Prot
P26433
Accession Number
NP_036786
Cellular Locus
Membrane
Host
Rabbit
Species Reactivity
Rat,Mouse
Immunogen
Synthetic peptide mapping to AA 809 to 831 of rat sequence
Target
NHE3
Clonality
Polyclonal
Conjugation
RPE
Tissue Specificity
Most abundant in colon and small intestine, followed by kidney and stomach. In kidney, expressed in proximal tubules and outer medulla (at protein level)
Validated Applications
WB,IHC,ICC/IF
Purification
Protein A Purified
Concentration
1 mg/ml
Dilution
WB (1:1000); IHC (1:25); optimal dilutions for assays should be determined by the user.
Weight
0.1
Buffer
95.64mM Phosphate, 2.48mM MES and 2mM EDTA
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Kim G.H., Ecelbarger C.A., Knepper M.A. and Packer R.K. (1999) J Am Soc Nephrol. 10: 935-942.
2. Dynia D.W., Steinmetz A.G. and Kocinsky H.S. (2010) Am J Physoil Renal Physiol. 298(3): F745-F753.
Product Datasheet
https://cdn.gentaur.com/products/400/4414056/datasheet/spc-400d-rpe.pdf
Product MSDS
https://cdn.gentaur.com/products/400/4414056/msds/spc-400d-rpe.pdf
CAS Number
9007-83-4
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