Rab5 Antibody: ATTO 488

Rabbit Anti-Human Rab5 Polyclonal

Product Specifications

Background

Rab5 is a 24 kDa GTPase that regulates early endosome dynamics and endocytic trafficking. It is ubiquitously expressed and localizes to early endosomes and the plasma membrane. Rab5 controls the fusion of endocytic vesicles with early endosomes and mediates homotypic endosome fusion. It recruits effectors such as EEA1 and Rabaptin-5 to facilitate vesicle docking and fusion. In the nervous system, Rab5 is essential for receptor internalization, synaptic vesicle recycling, and neurotrophin signaling. Dysregulation of Rab5 activity can lead to endosomal dysfunction, impaired signaling, and neurodegeneration. It is widely used as a marker of early endosomes and is a key player in maintaining neuronal homeostasis.

CAS Number

9007-83-4

Specifications

Detects ~26kDa.

Product Name Alternative

Rab 5A, RAS associated protein RAB5A, Ras related protein Rab 5 A

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

5868

Swiss Prot

P20339

Accession Number

NP_004153.2

Cellular Locus

Cell Membrane | Endosome | Early Endosome Membrane | Melanosome

Host

Rabbit

Species Reactivity

Human,Mouse,Rat,Bovine,Monkey

Immunogen

Human Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues

Target

Rab5

Clonality

Polyclonal

Conjugation

ATTO 488

Type

Polyclonal

Applications

WB | IHC | ICC/IF

Validated Applications

IHC,ICC/IF

Field of Research

Purification

Protein A Purified

Detection Range

1 µl/ml of SPC-168 was sufficient for detection of Rab5 in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:100), ICC/IF (1:80) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Sonnichsen B., et al. (2000) J. Cell Biol. 149: 901-913 6. Woodman P.G. (2000) Traffic. 1: 695-701.