Methylated Lysine Antibody: HRP

Rabbit Anti-Methylated Lysine Polyclonal

Product Specifications

Background

Methylated lysine is a key post-translational modification that plays a pivotal role in regulating gene expression, chromatin structure, and protein-protein interactions in the nervous system. Lysine residues on histone proteins can undergo mono-, di-, or tri-methylation, influencing transcriptional activation or repression depending on the site and degree of methylation. This epigenetic regulation is essential for neuronal development, synaptic plasticity, and memory formation. In neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s disease, aberrant lysine methylation patterns have been linked to dysregulated gene expression and impaired neuronal function. Enzymes that catalyze lysine methylation (lysine methyltransferases) and demethylation (lysine demethylases) are increasingly recognized as contributors to disease pathogenesis. Altered activity of these enzymes can disrupt chromatin dynamics, leading to neuronal vulnerability and degeneration. Targeting lysine methylation pathways offers a promising therapeutic strategy for modulating gene expression and restoring neuronal homeostasis. As research advances, methylated lysine serves as both a biomarker and a mechanistic link between epigenetic regulation and neurodegenerative progression.

CAS Number

9007-83-4

Specifications

Detects proteins containing methylated lysine residues.

Product Name Alternative

Methylated Lysine, Methyl lysine, Dimethylysine, Trimethyl lysine, N epsilon dimethyl lysine

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Host

Rabbit

Species Reactivity

Species Independent

Immunogen

Methylated KLH Conjugated

Target

Methylated Lysine

Clonality

Polyclonal

Conjugation

HRP

Type

Polyclonal

Applications

WB | ICC/IF | IP | ELISA

Validated Applications

WB,IHC,ELISA

Field of Research

Cell Signaling | Post-translational Modifications | Methylation

Purification

Protein A Purified

Detection Range

0.2-0.5 µg/ml of SPC-160 was sufficient for detection of the methylated histone by western blot analysis using melanoma cells in TBSt.

Concentration

0.25 mg/ml

Dilution

WB (1:5000), ICC/IF (1:50) ; optimal dilutions for assays should be determined by the user.

Weight

0.4

Buffer

73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Yang X.J. (2005) Oncogene. 24:1653-1662. 2. Hassig C.A. and Schreiber S.L. (1997) Curr. Opin. Chem. Biol. 1 (3) : 300-308. 3. Yang X.J. (2004) Bioessays 26:1076-1087. 4. Hughes R.E. (2002) Curr. Biol. 12: R141-R143. 5. Vigushin D.M. and Coombes R.C. (2004) Curr. Cancer Drug Targets 4: 205-218. 6. Chan H.M. et al. (2001) Nat. Cell Biol. 3: 667-674. 7. Martinez-Balbas M.A. et al. (2000) EMBO J. 19: 662-671.