Phosphothreonine Antibody: ATTO 488

Rabbit Anti-Phosphothreonine Polyclonal

Product Specifications

Background

Phosphothreonine is a phosphorylated derivative of threonine, catalyzed by serine/threonine kinases. It serves as a regulatory switch in numerous signaling pathways that govern neuronal survival, synaptic function, and stress responses. In the brain, phosphothreonine modifications influence the activity of key proteins such as tau, MAPKs, and transcription factors. Dysregulated threonine phosphorylation is associated with protein misfolding, impaired axonal transport, and neuroinflammation—hallmarks of neurodegenerative diseases. Notably, abnormal phosphorylation of tau at threonine residues contributes to its aggregation and toxicity in Alzheimer’s disease. As a modifiable and site-specific event, phosphothreonine is a valuable marker for disease progression and a potential target for kinase-based therapies in neurodegeneration.

CAS Number

9007-83-4

Specifications

Detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.

Product Name Alternative

O-phospho-L-threonine, Phospho-threonine

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Host

Rabbit

Species Reactivity

Species Independent

Immunogen

Phosphothreonine conjugated to KLH

Target

Phosphothreonine

Clonality

Polyclonal

Conjugation

ATTO 488

Type

Polyclonal

Applications

WB | ICC/IF | IP | ELISA

Validated Applications

IHC,ICC/IF

Field of Research

Cell Signaling | Post-translational Modifications | Phosphorylation

Purification

Peptide Affinity Purified

Detection Range

2 µg/ml of SPC-154 was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium.

Concentration

0.25 mg/ml

Dilution

WB (1:500), ICC/IF (1:60), ELISA (1:2000), IP (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.4

Buffer

640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Ostrovsky P.C. (1995) Genes Dev. 9 (16) : 2034-2041.