Phosphothreonine Antibody

Rabbit Anti-Phosphothreonine Polyclonal

Product Specifications

Background

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).

Specifications

Detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.

Product Name Alternative

Cell Signaling, Post-translational Modifications, Phosphorylation

UNSPSC

12352203

Host

Rabbit

Species Reactivity

Species Independent

Immunogen

Phosphothreonine conjugated to KLH

Target

Phosphothreonine

Clonality

Polyclonal

Conjugation

Unconjugated

Validated Applications

WB,ICC/IF,IP,ELISA

Purification

Peptide Affinity Purified

Concentration

0.25 mg/ml

Dilution

WB (1:500), ICC/IF (1:60), ELISA (1:2000), IP (1:100); optimal dilutions for assays should be determined by the user.

Weight

0.4

Buffer

PBS, 50% glycerol, 0.01% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Ostrovsky P.C. (1995) Genes Dev. 9(16): 2034-2041.