Phosphoserine Antibody: ATTO 390

Rabbit Anti-Phosphoserine Polyclonal

Product Specifications

Background

Phosphoserine is a post-translationally modified form of the amino acid serine, resulting from phosphorylation by serine/threonine kinases. This modification plays a critical role in regulating protein function, localization, and interactions, particularly in the central nervous system. In neurons, phosphoserine is involved in signal transduction, synaptic plasticity, and cytoskeletal dynamics. Aberrant serine phosphorylation has been implicated in the pathogenesis of neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and ALS. For example, hyperphosphorylation of tau protein at serine residues contributes to neurofibrillary tangle formation and neuronal dysfunction in Alzheimer’s disease. Phosphoserine also modulates the activity of transcription factors, ion channels, and enzymes involved in oxidative stress responses and apoptosis. As a dynamic and reversible modification, it offers a promising target for therapeutic intervention and biomarker development in neurodegeneration.

CAS Number

9007-83-4

Specifications

Detects proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.

Product Name Alternative

O-phospho-L-serine, Phospho-serine, OPS, Phospho-ser, pS, pSer

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Host

Rabbit

Species Reactivity

Species Independent

Immunogen

Phosphoserine conjugated to KLH, and phosvitin mixture

Target

Phosphoserine

Clonality

Polyclonal

Conjugation

ATTO 390

Type

Polyclonal

Applications

WB | IHC | ICC/IF | IP | ELISA

Validated Applications

IHC,ICC/IF

Field of Research

Cell Signaling | Post-translational Modifications | Phosphorylation

Purification

Peptide Affinity Purified

Detection Range

2 µg/ml of SPC-149 was sufficient for detection of phosphorylation signal in western blot analysis using human MMRU cells treated with 0.1 µM okadaic acid.

Concentration

0.25 mg/ml

Dilution

WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.4

Buffer

640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Ostrovsky P.C. (1995) Genes Dev. 9 (16) : 2034-2041.