Rab4 Antibody

Rabbit Anti-Human Rab4 Polyclonal

Product Specifications

Background

Rab4 is a 25 kDa GTPase of the Ras superfamily that regulates early endosomal recycling. It cycles between an inactive GDP-bound state and an active GTP-bound state, recruiting effector proteins to intracellular membranes. Rab4 modulates receptor recycling, including transferrin and glucose transporter (GLUT4) trafficking. Overexpression of Rab4 alters receptor distribution, leading to accumulation in post-endosomal compartments. Its activity is regulated by phosphorylation and post-translational lipid modifications, which influence membrane association and subcellular localization. In neurons, Rab4 is involved in synaptic vesicle recycling and receptor trafficking. Dysregulation of Rab4 may impair endosomal sorting and contribute to synaptic dysfunction in neurodegenerative diseases such as Alzheimer’s and Parkinson’s.

CAS Number

9007-83-4

Specifications

Detects ~26kDa.

Product Name Alternative

Oncogene Rab4, Rab4A, Ras related protein

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

5867

Swiss Prot

P20338

Accession Number

NP_004569.2

Cellular Locus

Membrane | Cytoplasm

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

C-terminal peptide from human Rab4

Target

Rab4

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal

Applications

WB | IHC | ICC/IF

Validated Applications

WB,IHC,ICC/IF

Field of Research

Purification

Peptide Affinity Purified

Detection Range

A 1:1000 dilution of SPC-141 was sufficient for detection of Rab4 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:100), ICC/IF (1:150) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Cormont M., et al. (1996) Mol Cell Biology. 16: 6879-6886. 6. Ayad N., Hull M., and Mellman I. (1997) EMBO 16: 4497-4507. 7. Van der Sluijs, P. et l. (1992) The EMBO Journal 11 (12), 4379-4389.