PDI Antibody

Rabbit Anti-Rat PDI Polyclonal

Product Specifications

Background

Protein disulfide isomerase (PDI) is a thiol-disulfide oxidoreductase that facilitates protein folding and quality control in the endoplasmic reticulum (ER) . In neurons, PDI plays a protective role by preventing the accumulation of misfolded proteins and maintaining redox homeostasis. Under conditions of ER stress—common in neurodegenerative diseases—PDI expression is upregulated as part of the unfolded protein response (UPR) . However, excessive or dysfunctional PDI activity has been linked to protein aggregation and oxidative damage in Alzheimer’s, ALS, and Parkinson’s disease. PDI’s dual role in neuroprotection and pathology makes it a compelling target for therapeutic strategies aimed at modulating ER stress and proteostasis in neurodegeneration.

CAS Number

9007-83-4

Specifications

Detects ~58kDa.

Product Name Alternative

Thioredoxin domain containing 5, ERP46, UNQ364, MGC3178, FLJ21353, FLJ90810, thioredoxin related protein, endothelial protein disulphide isomeras

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Swiss Prot

P04785

Cellular Locus

Endoplasmic Reticulum | Endoplasmic Reticulum Lumen

Host

Rabbit

Species Reactivity

Human,Mouse,Rat,Bovine,Dog,Hamster,Guinea Pig,Sheep,Pig,Xenopus,Mollusk

Immunogen

AA499-509 of Rat PDI

Target

PDI

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal

Applications

WB | IHC | ICC/IF | IP

Validated Applications

WB,IHC,ICC/IF,IP

Field of Research

Cell Signaling | Protein Trafficking | Chaperone Proteins | Tags and Cell Markers | Organelle Markers

Purification

Protein A purified

Detection Range

A 1:1000 dilution of SPC-114 was sufficient for detection of PDI in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. NA K.S. et al. (2007) Mol Cells. 24 (2) : 261-7. 2. Mayer M., Kies U., Kammermeier R., and Buchner J. (2000) J Biol Chem. 275 (38) : 29421-5. 3. Delom F., Mallet B., Carayon P., and Lejeune P.J. (2001) J Biol Chem 276 (24) : 21337-42. 4. Schultz-Norton J.R., McDonald W.H., Yates J.R. and Nardulli A.M. (2006) Mol Endocrinol 20 (9) : 1982-95. 5. Turano C., Coppari S. Altieri F. and Ferraro (2002) J Cell Physiol 193: 154-163. 6. Janiszewski M. (2005) J. Biol Chem. 280 (49) : 40813- 40819. 7. Yoshimori T., et al. (1990) J Biol Chem. 265 (26) : 15984-90.