Mouse LIF Protein, Tag Free (MALS verified)
Mouse LIF Protein, Tag Free (LIF-M5219) is expressed from human 293 cells (HEK293) . It contains AA Ser 24 - Phe 203 (Accession # P09056-1) .
Product Specifications
Background
Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
Specifications
This protein carries no "tag". The protein has a calculated MW of 20.0 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P09056-1
Expression Region
Ser 24 - Phe 203
Host
HEK293
Target
LIF
Conjugation
Unconjugated
Tag
Native
Source
Mouse
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
0.1 EU per μg
Purity
90%
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
20.0 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
1mg*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/LIF-M5219.pdf
Species
Mouse
Curated Selection
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