Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His, Avitag™&Flag Tag (MALS verified)

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Product Specifications

Background

Specifications

Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His, Avitag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 17.7 kDa (CD3E) and 15.4 kDa (CD3G) . Subunit CD3E carries a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 19-20 kDa and 22-24 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE) .

Accession Number

Q95LI5-1 (CD3E) & Q95LI7-1 (CD3G)

Expression Region

Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G)

Host

HEK293

Target

CD3E & CD3G

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi | C-Flag

Source

Cynomolgus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Bispecific BCMA×CD3 T cell-engaging Antibody at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-C8219) with a linear range of 2.4-78 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

17.7 kDa (CD3E) & 15.4 kDa (CD3G)

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Storage Conditions

-20°C

Package Size

200ug*1

Host or Source

HEK293

Species

Cynomolgus

Protein ID

Q95LI5-1 (CD3E) & Q95LI7-1 (CD3G)

Preservative

Trehalose

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