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Mouse anti Human Bence Jones kappa (surface and hidden determinants)

Product Specifications

Background

The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidase staining and immunoblotting. The optimum working dilution is an assayrelated characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

CAS Number

9007-83-4

Host

Mouse

Isotype

IgG1

Clone

NI 285, NI 250

Source

Highly purified Bence Jones kappa proteins isolated from pooled Human urine.

Assay Principle

Indirect immunofluorescence,ELISA.

Form

Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7,2); Nopreservative added, as it may interfere with the antibody activity; Noforeign protein added IgG concentration is 0,4 mg/mL No foreign proteins added

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Storage Conditions

The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day
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