Human 2B4 / CD244 / SLAMF4 Protein, Llama IgG2b Fc Tag, low endotoxin

Human 2B4, Llama IgG2b Fc Tag (2B4-H5256) is expressed from human 293 cells (HEK293). It contains AA Cys 22 - Arg 221 (Accession # Q9BZW8-2).

Product Specifications

Background

Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contains two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.

Specifications

This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 50.1 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Cys 22 - Arg 221

Host

HEK 293

Target

2B4

Conjugation

Unconjugated

Tag

C-LlamaFc(LlamaIgG2b)

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

95%

Bioactivity

Immobilized Human 2B4, Llama IgG2b Fc Tag (Cat. No. 2B4-H5256) at 5 μg/mL (100 μL/well) can bind Human CD48, His Tag (Cat. No. BC1-H5226) with a linear range of 0.02-0.625 μg/mL (QC tested).