Fast Green FCF

Fast Green FCF is a sea green triarylmethane food dye, with absorption maximum ranging from 622 to 626 nm. Fast Green FCF inhibits α-synuclein aggregation, as well as Aβ and P2X4 receptor, and TLR4/Myd88/NF-κB. Fast Green FCF is widely used as a staining agent like quantitative stain for histones at alkaline pH after acid extraction of DNA, and as a protein stain in electrophoresis. Fast Green FCF improves cognitive impairment, depression, relieves pain allergies, and promotes reproductive function[1][2][3][4][5][6][7][8][9].

Product Specifications

CAS Number

[2353-45-9]

Product Name Alternative

FD&C Green No. 3; Food green 3; C.I. 42053

UNSPSC

12171500

Hazard Statement

H335, H341

Target

α-synuclein

Type

Dye Reagents

Related Pathways

Neuronal Signaling

Field of Research

Neurological Disease

Assay Protocol

https://www.medchemexpress.com/Fast-Green-FCF.html

Concentration

10mM

Purity

99.95

Solubility

H2O : 10 mg/mL (ultrasonic)

Smiles

O=S(C1=CC(C/[N+](CC)=C2C=C/C(C=C/2)=C(C3=CC=C(N(CC)CC4=CC=CC(S(=O)(O[Na])=O)=C4)C=C3)\C5=CC=C(O)C=C5S(=O)(O[Na])=O)=CC=C1)([O-])=O

Molecular Formula

C37H34N2Na2O10S3

Molecular Weight

808.85

Precautions

H335, H341

References & Citations

[1]Mittal A, et al. Batch and bulk removal of a triarylmethane dye, Fast Green FCF, from wastewater by adsorption over waste materials. J Hazard Mater. 2009 Apr 30;163 (2-3) :568-77. |[2]How SC, et al. Examining the inhibitory potency of food additive fast green FCF against amyloid fibrillogenesis under acidic conditions. Food Funct. 2016 Dec 7;7 (12) :4898-4907.|[3]van Hooft JA. Fast Green FCF (Food Green 3) inhibits synaptic activity in rat hippocampal interneurons. Neurosci Lett. 2002 Feb 1;318 (3) :163-5.|[4]Wang F, et al. The food additive fast green FCF inhibits α-synuclein aggregation, disassembles mature fibrils and protects against amyloid-induced neurotoxicity. Food Funct. 2021 Jun 21;12 (12) :5465-5477.|[5]Liu F, et al. Fast green FCF inhibits Aβ fibrillogenesis, disintegrates mature fibrils, reduces the cytotoxicity, and attenuates Aβ-induced cognitive impairment in mice. Int J Biol Macromol. 2021 Feb 15;170:33-41.|[6]Liu J, et al. Fast green FCF prevents postoperative cognitive dysfunction via the downregulation of the P2X4 receptor in mice. Int Immunopharmacol. 2023 Aug;121:110462.|[7]Yang J, et al. Fast Green FCF Attenuates Lipopolysaccharide-Induced Depressive-Like Behavior and Downregulates TLR4/Myd88/NF-κB Signal Pathway in the Mouse Hippocampus. Front Pharmacol. 2019 May 8;10:501.|[8]Xu F, et al. Fast Green FCF Alleviates Pain Hypersensitivity and Down-Regulates the Levels of Spinal P2X4 Expression and Pro-inflammatory Cytokines in a Rodent Inflammatory Pain Model. Front Pharmacol. 2018 May 23;9:534.|[9]Dixit A, et al. ASSESSMENT OF REPRODUCTIVE TOXICITY CAUSED BY FAST GREEN FCF ON MALE SWISS ALBINO MICE. 2012.