Biotinylated Mouse IL-17B Protein, Fc, Avitag™ (MALS verified)

IL-17B is a member of IL17 famaily, was originally described as a proinflammatory cytokine. IL-17B is produced by neutrophils and potentiates TNF-α effect on the production of cytokines and chemokines, such as IL-6, G-CSF, and CCL20, known to control immune cell trafficking to inflamed tissues. Interestingly, although IL-17B and IL-17E (IL-25) share a common receptor, IL-17RB, IL-17B, and IL-17E deficiency lead to opposite results in a model of acute colitis induced by dextran sulfate sodium. IL-17B inhibits IL-17E binding to IL-17RA– IL-17RB complexes on epithelial cells, and limits IL-17E-induced IL-6 production by colon epithelial cells.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 46.0 kDa. The protein migrates as 55-58 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q9QXT6

Expression Region

Arg 23 - Phe 180

Host

HEK293

Target

IL-17B

Conjugation

Biotin-labeled

Tag

C-Fc & C-Avi

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Mouse IL-17B Protein, Fc,Avitag (Cat. No. ILB-M82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Human IL17B Monoclonal Antibody with a linear range of 5-78 ng/mL (Routinely tested).