NBD-F

NBD-F (4-Fluoro-7-nitrobenzofurazan) is a pro-fluorescent reagent which is developed for amino acid analysis. NBD-F reacts with primary or secondary amines to produce a fluorescent product and used for analysis of amino acids and low molecular weight amines[1].

Product Specifications

CAS Number

[29270-56-2]

Product Name Alternative

4-Fluoro-7-nitrobenzofurazan

UNSPSC

12171500

Hazard Statement

H315, H319

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/NBD-F.html

Concentration

10mM

Purity

99.68

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

O=[N+](C1=CC=C(F)C2=NON=C21)[O-]

Molecular Formula

C6H2FN3O3

Molecular Weight

183.10

Precautions

H315, H319

References & Citations

[1]Wu X, et al. Determination of amino acid neurotransmitters in rat hippocampi by HPLC-UV using NBD-F as a derivative. Biomed Chromatogr. 2014 Apr;28 (4) :459-62.|[2]Ishikawa T, et al. Development of an LC-MS/MS method for the analysis of free sphingoid bases using 4-fluoro-7-nitrobenzofurazan (NBD-F) . Lipids. 2014 Mar;49 (3) :295-304.