Recombinant Human Aldehyde dehydrogenase X, mitochondrial (ALDH1B1), partial

Product Specifications

Product Name Alternative

(Aldehyde dehydrogenase 5) (Aldehyde dehydrogenase family 1 member B1)

Abbreviation

Recombinant Human ALDH1B1 protein, partial

Gene Name

ALDH1B1

UniProt

P30837

Expression Region

167-264aa

Organism

Homo sapiens (Human)

Target Sequence

SRVISQPDQTLEILMSELDPAVMDQFYMKDGVTAKDVTRESGIRDLIPGSVIDATMFNPCGYSMNGMKSDGTYWTIHITPEPEFSYVSFETNLSQTSY

Tag

N-terminal 10xHis-GST-tagged and C-terminal V5-Myc-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Metabolism

Relevance

ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

47.6 kDa

References & Citations

"Aldehyde dehydrogenase 1B1: molecular cloning and characterization of a novel mitochondrial acetaldehyde-metabolizing enzyme." Stagos D., Chen Y., Brocker C., Donald E., Jackson B.C., Orlicky D.J., Thompson D.C., Vasiliou V. Drug Metab Dispos 38:1679-1687 (2010)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.