AAV-DJ Helper Free Promoterless Expression System

The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1) . AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2.

Product Specifications

Overview

Complete Expression System contains packaging plasmids, expression vector and GFP control vector/pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus/AAV-DJ system provides a hybrid capsid created from 8 different AAV serotypes to provide substantially higher infectivity rates across a broad range of tissues/Cloning capacity of expression vector = 3.9 kb

UNSPSC

41105908

Features & Benefits

Complete Expression System contains packaging plasmids, expression vector and GFP control vector; pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus; AAV-DJ system provides a hybrid capsid created from 8 different AAV serotypes to provide substantially higher infectivity rates across a broad range of tissues

Shipping Conditions

Blue Ice

Storage Conditions

-20ºC

Notes

FOR SALE TO ACADEMIC, GOVT AND NON-PROFIT LABS ONLY

Classification

Viral Expression->Adeno-Associated Virus (AAV) Expression->AAV Expression and Packaging->AAV Helper Free Complete Expression Systems->AAV-DJ Helper Free Complete Expression Systems