QuickTiter™ HBV Core Antigen ELISA Kit
An anti-HBVcAg monoclonal coating antibody is adsorbed onto a microtiter plate. HBV core antigen present in the sample or standard binds to the antibodies adsorbed on the plate; a FITC-conjugated mouse anti-HBVcAg antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-HBVcAg. Following unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of HBV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HBV core antigen and sample concentration is then determined.
Product Specifications
Overview
Measures the core antigen of Hepatitis B virus/Sensitive to as low as 1 ng/mL/Recombinant HBVcAg standard included/
CAS Number
7732-18-5
UNSPSC
41116158
Hazard Statement
Excepted Quantities
Detection Method
Colorimetric
Features & Benefits
Measures the core antigen of Hepatitis B virus; Sensitive to as low as 1 ng/mL; Recombinant HBVcAg standard included
Shipping Conditions
6 parts: 5 RT / 1 Blue Ice
Storage Conditions
Upon receiving, aliquot and store recombinant HBVcAg Standard at -20ºC and avoid freeze/thaw. Store all other components at 4ºC.
Classification
Pathogen and Toxin Assays->Virus Core Antigen Detection->HBV Core Antigen ELISA
lmo Content
Recombinant Protein purified from E. coli
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