OxiSelect™ In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Colorimetric)
Cell Biolabs’ OxiSelect™ Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO2-/NO3- determination. First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme. Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm) . Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 µM (in 50 µL sample volume) .
Product Specifications
Overview
Measure NO in various samples by NO2-/NO3-determination/Suitable for serum, plasma, urine, saliva, lysates, and media
UNSPSC
41116100
Hazard Statement
Excepted Quantities
Detection Method
Colorimetric
Features & Benefits
Measure NO in various samples by NO2-/NO3-determination; Suitable for serum, plasma, urine, saliva, lysates, and media;
Shipping Conditions
Dry Ice
Storage Conditions
Store the entire kit at -80ºC
Classification
Oxidative Stress/ Damage->Reactive Oxygen Species (ROS) Assays->In Vitro Nitric Oxide Assay
lmo Content
None
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