ROCK Activity Immunoblot Kit
Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20) . Our ROCK Activity Immunoblot Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates.
Product Specifications
Overview
Safe non-radioactive Western blot assay format
UNSPSC
41116100
Detection Method
Western Blot
Features & Benefits
Safe non-radioactive Western blot assay format;
Shipping Conditions
Blue Ice
Storage Conditions
Store active ROCK-II at -80ºC and all other kit components at -20ºC. Avoid multiple freeze/thaw cycles.
Classification
Cell Signaling and Protein Biology->Kinase Assays->Rho Kinase (ROCK) Assays
lmo Content
Recombinant Protein from Baculovirus
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