Global DNA Methylation ELISA Kit (5’-methyl-2’-deoxycytidine Quantitation)
The Global DNA Methylation ELISA Kit is a competitive ELISA for the quantitative measurement of 5-methylcytosine (5MedCyd) . The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.
Product Specifications
Overview
For detection and quantitation of 5'-methyl-2'-deoxycytidine (5MedCyd) /Suitable for use with DNA samples such as cell or tissue genomic DNA/Detection sensitivity of 150 nM 5MedCyd/
CAS Number
9000-83-3
UNSPSC
41116158
Hazard Statement
Excepted Quantities
Detection Method
Colorimetric
Features & Benefits
For detection and quantitation of 5'-methyl-2'-deoxycytidine (5MedCyd); Suitable for use with DNA samples such as cell or tissue genomic DNA; Detection sensitivity of 150 nM 5MedCyd
Shipping Conditions
6 parts: 5 RT / 1 Blue Ice
Storage Conditions
Upon receipt, aliquot and store the 5MedCyd Standard and 5MedCyd DNA Conjugate at -20ºC to avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
Classification
Oxidative Stress/ Damage->DNA / RNA Damage and Repair->Global DNA Methylation and Hydroxymethylation
lmo Content
None
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